- Epidemiological and Bacteriological Examination on the Cholera of Taegu Area in 1970
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Jae Sik Kim, Hae Jin Kim, Jyung Myung Kim
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Korean J Cytopathol. 1971;5(1):25-31.
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 Abstract
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- The cholera prevailed at Taegu, Korea in August, 1970 was analysed for the epidemiological and bacteriological determinations from true and suspicious, total 109, cases which were admitted to Kyungpook National University Hospital.
The results were as followings:
1. The isolation rate of cholera was 33.0% (36 cases) from stool culture.
2. The incidence on sex and age showed that the female group was more than 2 times higher than the male group and more high in twenties group in the female but thirties in the male group.
3. The consistencies of the feces were rice watery in 7 cases (19.4%), diarrheal in 25 cases (65.5%) and formular in 4 cases (11.1%).
4. The excretion of bacteria in feces was continued to the 5∼6th day in most cases, however there was no case continuing the excretion of bacteria from feces beyond 10th day.
5. All of the true cases were the inhibitants of peripheral area of the city or country near the city.
6. The suspected intermediate as an infectious source was generally likely to be sea fishes, fruits and sea plant etc. in order but it was impossible to determine the primary source of the infection.
7. The isolated organism was serologically Ogawa type and revealed positive indole test, Voges-Proskauer test, cholera red test, hemolytic reaction and hemagglutination test on hen red cells in most strains and were resistant to phage type IV in all strains tested.
On the carbohydrate fermentation test, glucose and sucrose were fermented but lactose, mannitol, maltose and arabinose were not fermented in most strains.
The sensitivity test to the various antibiotics revealed high sensitivity to streptomycin, chloramphenicol, eythromycin and penbritin but there was a tendency of resistance to tetracyclin is tested strains.
- Studies on Rapid Fractionation of Serum Protein by Salting Out Method
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Jae Sik Kim, Hae Kyo Hong
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Korean J Cytopathol. 1971;5(1):33-37.
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Abstract
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- A study on rapid fractionation of the serum protein by salting out method rather than expensive electrophoresis was performed with standard normal serum and 100 sera of the normal persons as an evaluation of the usefulness in the screening or replacent of the paper electrophoretic procedure.
The results were as followings:
1. The filtration procedure was less time consuming and more simple than surface active agent, span ether, treatment in albumin fractionation.
2. The values obtained from the 100 sera of normal persons were 6.99±0.49gm% in the total protein, 4.13±0.37 gm% in the albumin, 2.86±0.35gm% in the total globulin, 0.95±0.21gm% in the alpha globulin, 0.92±0.29gm% in the beta globulin, 0.99±0.24gm% in the gamma globulin and 1.44±0.25 in the A-G ratio, while the standard normal serum revealed 6.60gm% in the total protein, 3.80gm% in the albumin, 2.80 gm% in the total globulin, 0.80gm% in the alpha globulin, 1.17 gm% in the beta globulin 0.93gm% in the gamma globulin and 1.35 in the A-G ratio.
3. From the above results, it see%ed to be approximated to the values of the paper electrophoresis of another authors.
- Total Protein, Paper Electrophoretic Patterns, CRP Reaction and Hemoglobin Content in Lepromatous Leprosy
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Jae Sik Kim, Kae Nam Kim, Seong Jik Yoon, Jae Hwan Kim, Jyung Myung Kim
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Korean J Cytopathol. 1970;4(2):117-121.
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Abstract
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- Total protein, paper electrophoretic patterns, CRP reaction and hemoglobin in lepromatous leprosy cases were tested to estimate recent evaluations compare to past reports.
1. Total protein was 6.99±0.53 gm% in mean value and this result was similar to past reports.
2. In findings of the electrophoretic patterns albumin and α1-, α2-, β-, and γ-globulin were 47.38±0.86%, 6.48±0.94%, 9.54±0.38%, 13.13 ±0.54% and 23.38±0.65% of total protein. From this result of protein fractions, it showed decreased albumin and increased globulin fractions which resulted in decrease of A/G ratio and this result is nearly as same as past reports.
3. There were only 4 cases (8.33%) of positive CRP reaction.
4. The mean value of hemoglobin content was 12.43±0. l7gm%.
- The Determination of Blood Sugar by Glucose Oxidase Method
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Jae Sik Kim, Seong Jik Yoon, Hi Sook Park, Joon Shik Park, Bok Duk Oh
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Korean J Cytopathol. 1969;3(1):9-12.
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- Since high degree of specificity of glucose oxidase for beta-glucose had been demonstrated, the use of the enzyme, glucose oxidase and peroxidase coupled with a chromogenic oxygen acceptor such as O-dianisidine for the colorimetric determination of glucose in body fluid was proposed from several investigators. In this report the glucose level was determined in the non-and deproteinized serum and blood by means of glucose oxidase method of the Sigma Company, also the recovery rate was examined by addition of the standard glucose compatible to 50 to 100mg% with following results :
1. It was found that this method was available in the determination of blood sugar without deproteinization in nonhemolytic serum.
2. When the known amounts of glucose solution were added to the serum and blood, the recoveries were ranged from 90 to 110%.
- Scotochromogens From Tuberculous Patients
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Jae Sik Kim, Won Bae Kim, Jae Joo Suh, Yong Ma Hah, Young Myung Kim
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Korean J Cytopathol. 1968;2(1):39-43.
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- The incidence of mycobacterium infection other than tubercle bacilli from tuberculous patients has been reported increasingly in recent years and the significance of unclassified mycobacteria in clinical cases evaluated intensively.
In the course of the studies for the distribution of unclassified mycobacteria from tuberclous patients and other sources, authors isolated 29 strains of scotochromogens and tested biochemical characters.
1) Nine strains were isolated from 25 cases of tuberculous patients in mineral industry and 20 strains from various sources of patient from university hospital.
2) All strains isolated were belong to scotochromogens by biochemical characteristics exhibiting following characters; color formation in darkness, strong catalase positive, nitrate reduction negative except one strain and urease positiveness in 24 strains.
3) Growth rate were rather slow grower in Ogawa media forming colonies between 10 and 14 days and sensitivity pattern to antituberculous agents exhibited an usual pattern of unclassified mycobacteria in that test strains were moderately sensitive to streptomycin and isonicotinic acid hydrazide, whereas these were resistant to paraaminosalicylic acid.
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